Miscellaneous Services & Supplies Serviceġ Usage fee applies to the Covaris Ultrasonicator, Qiagen TissueLyzer, Synergy H1 Hybrid Plate Reader, and GenoGrinder 2010 instruments. We cannot guarantee customer-supplied libraries will meet this estimate.ģ Lane or block pricing reflects divided user costs, however sequencing turn-around times are subject to sufficient user volume in order to fill the flow cell.Ĥ Block sequencing is performed on NovaSeq S4 150 PE lanes, and is only available for select GSL-prepared library options, we cannot offer block pricing options for user prepared libraries, or those not suitable for sequencing at 150×2 PE, or will impact the performance of other customer libraries. Please see our Bioanalyzer & Tapestation pricing for more information.Ģ Yield estimates reflect optimum values defined by Illumina and apply to GSL prepared libraries only. Per cell (10.0 Billion reads 2) – 4 independent lanesġ For GSL library preparation, an additional charge for template quality & integrity will be added. Per cell (4.0 Billion reads 2) – 2 independent lanes NovaSeq 6000 S2 100 bp SR/50 PE flow cell Per cell (1.6 Billion reads 2) – 2 independent lanes NovaSeq 6000 S1 100 bp SR/50 PE flow cell Per cell (800M reads 2) – 2 independent lanes ![]() NovaSeq 6000 SP 100 bp SR/50 bp PE flow cell Per sample Sequencing (NovaSeq S4 150 PE) NextSeq 500 v2 150 bp SR/ 75 bp PE flow cell Next-Generation Sequencing Illumina Services Serviceġ0X Genomics 3′ SC RNA library prep (internal users only, BSL1 only) Gold Sequencing – Seq Rxn, Clean Up & Capillary Runīronze Sequencing – Clean Up & Capillary Run Platinum Sequencing – PCR clean up (ExoSap), Seq Rxn, Clean Up & Capillary Run Sanger Sequencing & Genotyping 96-well plate Service Bioanalyzer RNA chips run 11-12 samples, depending on the sensitivity level (pico vs nano). TapeStation High Molecular Weight DNA AnalysisīioAnalyzer RNA Analysis – Pico sensitivityīioAnalyzer RNA Analysis – Nano sensitivityġ Split pricing is available for half (1-6) and full (7-12) sets of samples. TapeStation High Sensitivity DNA Analysis TapeStation DNA Analysis (D1000/D5000 Tapes) Note: GSL DNA extraction services may not be suitable for NGS applications requiring extremely high molecular weight DNA inputs, such as PacBio or Oxford Nanopore long-read sequencing approaches. bcl file delivery available due to specific demultiplexing requirements. Due to unique cycle settings, 10X libraries require a dedicated flow cell for sequencing. Notes: 10X library prep is currently only available for internal users, utilizing cells with BSL1 (nonpathogenic) status. We will try and point you to a suitable provider if we are unable to provide services directly.Ĭurrent GSL Services & Pricing (effective April 3rd, 2023) Please contact the GSL Director BEFORE submitting ANY samples or research projects related to the SARS-COV2 pandemic or other samples of pathogenic risk to humans. The GSL is a University BSL-1 laboratory, classified as a teaching and training facility with student interactions. A standard 3% charge will be added for credit card transactions.
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